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- Description:
- The data set includes individual images of mouse cochleae, both scanning electron micrographs and fluorescent micrographs, used to generate aggregated data described in Pecha PP, Almishaal AA, Mathur PD, et al. Role of Free Radical Formation in Murine Cytomegalovirus–Induced Hearing Loss. Otolaryngology–Head and Neck Surgery. 2020;162(5):709-717. doi:10.1177/0194599820901485 and Objectives The goal of the study was to determine whether reactive oxygen species (ROS) mediates cytomegalovirus (CMV)–induced labyrinthitis. Study Design Murine model of CMV infection. Subjects and Methods Nrf2 knockout mice were inoculated with murine CMV. Auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAEs) were then performed on these and uninfected controls. BALB/c mice were inoculated with murine CMV to determine whether a marker for ROS production, dihydroethidium (DHE), is expressed 7 days after inoculation. Finally, 2 antioxidants—D-methionine and ACE-Mg (vitamins A, C, and E with magnesium)—were administered 1 hour before and after infection in inoculated mice for 14 days. Temporal bones were harvested at postnatal day 10 for DHE detection. ABR and DPOAE testing was done at postnatal day 30. Scanning electron microscopy was also performed at postnatal day 30 to evaluate outer hair cell integrity. Results Nrf2-infected mice had worse hearing than uninfected mice (P < .001). A statistically significant increase in DHE fluorescence was detected in BALB/c-infected mice as compared with uninfected mice 7 days after inoculation. D-methionine- and ACE-Mg-treated mice demonstrated an attenuation of the DHE fluorescence and a significant improvement in ABR and DPOAE thresholds when compared with untreated infected controls (P < .0001). Scanning electron microscopy demonstrated less outer hair cell loss in the treated versus untreated infected controls. Conclusion These results demonstrate for the first time that excessive ROS mediates CMV-induced hearing loss in a mouse model.
- Keyword:
- herpesviridae, hearing loss, cochlea, reactive oxygen species, cytomegalovirus, congenital CMV, mouse, outer hair cells, auditory brainstem response, and antioxidant
- Subject:
- cytomegalovirus and hearing loss
- Creator:
- Firpo, Matthew A., Park, Albert H., and Yang, Jun
- Owner:
- BRIAN MCBRIDE
- Based Near Label Tesim:
- Salt Lake City, Utah, United States
- Language:
- English
- Date Uploaded:
- 07/10/2019
- Date Modified:
- 05/31/2024
- Date Created:
- 2015-04-08 to 2016-11-02
- License:
- CC BY NC - Allows others to use and share your data non-commercially and with attribution.
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/10.7278/S50D-D0WT-SV00
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- Description:
- This dataset contains code used to generate and the results of 2D numerical modeling simulations of ambient resonance in damaged rock slopes. All simulations were performed using the Universal Distinct Element Code (UDEC) version 7.0. We simulated progressive damage for three different landslide types: slab toppling, flexural toppling, and planar sliding. For each scenario we simulated several stages of progressive rock slope damage. Subsequently, we recorded the resonance response of the rock slope at each stage by measuring x-direction velocity at one or more measuring points throughout the model.
- Subject:
- landslides, mathematical modeling, slope stability, and engineering geology
- Creator:
- Moore, Jeffrey R. and Jensen, Erin K.
- Owner:
- Jeff Moore
- Based Near Label Tesim:
- Utah, Utah, United States
- Language:
- English
- Date Uploaded:
- 10/18/2023
- Date Modified:
- 10/28/2023
- Date Created:
- 2023-01-01 to 2023-10-18
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/10.7278/S50d-j662-kdt9
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- Description:
- This dataset contains GIS map data and monitoring datasets collected between 2018 and 2022 at the Courthouse Mesa rock slope instability near Moab, Utah. Map data consist of an orthophoto, a polyline shapefile delineating mapped surficial cracks, and a point shapefile showing the locations of crack width monitoring points (M1–M5) and a vibrating wire crackmeter. Monitoring data include four years of continuous crack aperture measurements from the crackmeter, periodic crack width measurements from M1–M5, and three sets of air temperature measurements recorded between 2018 and 2022. Air temperatures were measured at the surface and inside the crack at several depths throughout the monitoring period.
- Subject:
- slope stability, engineering geology, geomorphology, and landslides
- Creator:
- Moore, Jeffrey R., Jensen, Erin K., Finnegan, Riley, and Geimer, Paul R.
- Owner:
- Erin Jensen
- Based Near Label Tesim:
- Moab, Utah, United States
- Language:
- English
- Date Uploaded:
- 07/19/2023
- Date Modified:
- 10/27/2023
- Date Created:
- 2018-01-01 to 2022-12-31
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/10.7278/S50d-e0es-913n
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- Description:
- Light-scattering spectroscopy (LSS) is an established optical approach for nondestructive characterization of biological tissues. Here, we investigated the capabilities of LSS and convolutional neural networks (CNNs) to quantitatively characterize the composition and arrangement of cardiac tissues. We assembled tissue constructs from 200 μm thick sections of fixed myocardium and aortic wall. Thickness of the tissue constructs was similar to the thickness of atrial free wall. In the assembled constructs, the aortic sections represented fibrotic tissue and the depth, volume fraction, and arrangement of these fibrotic insets were varied. We gathered spectra with wavelengths from 500-1100 nm from the constructs at multiple locations relative to a light source. We used single and combinations of two spectra for training of CNNs. With independently measured spectra, we assessed the accuracy of the trained CNNs for classification of tissue constructs from single spectra and combined spectra. In general, classification accuracy with single spectra was smaller than with combined spectra. Combined spectra including spectra from fibers distal from the illumination fiber typically yielded a higher accuracy than proximal single collection fibers. Maximal classification accuracy of depth detection, volume fraction and permutated arrangements was (mean±stddev) 88.97±2.49%, 76.33±1.51% and 84.25±1.88%, respectively. Our studies demonstrate the reliability of quantitative characterization of tissue composition and arrangements using a combination of LSS and CNNs. Potential clinical applications of the developed approach include intraoperative quantification and mapping of atrial fibrosis as well as assessment of ablation lesions.
- Subject:
- cardiology
- Creator:
- Hitchcock, Robert W., Sachse, Frank B., Cottle, Brian K., Kelson, Bailey E.B., and Knighton, Nathan J.
- Owner:
- Frank Sachse
- Based Near Label Tesim:
- Salt Lake City, Utah, United States
- Language:
- English
- Date Uploaded:
- 01/09/2020
- Date Modified:
- 01/22/2022
- Date Created:
- 20190101 to 20190208 and 20200721 to 20200807
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/10.7278/S50D-3Q4J-SC4Y
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- Description:
- Subglacial water pressures influence groundwater conditions in proximal alpine valley rock slopes, varying with glacier advance and retreat in parallel with changing ice thickness. Fluctuating groundwater pressures in turn increase or reduce effective joint normal stresses, affecting the yield strength of discontinuities. Here we extend simplified assumptions of glacial debuttressing to investigate how glacier loading cycles together with changing groundwater pressures generate rock slope damage and prepare future slope instabilities. Using hydromechanical coupled numerical models closely based on the Aletsch Glacier valley in Switzerland, we simulate Late Pleistocene and Holocene glacier loading cycles including long-term and annual groundwater fluctuations. Measurements of transient subglacial water pressures from ice boreholes in the Aletsch Glacier ablation area, as well as continuous monitoring of bedrock deformation from permanent GNSS stations helps verify our model assumptions. While purely mechanical glacier loading cycles create only limited rock slope damage in our models, introducing a fluctuating groundwater table generates substantial new fracturing. Superposed annual groundwater cycles increase predicted damage. The cumulative effects are capable of destabilizing the eastern valley flank of our model in toppling-mode failure, similar to field observations of active landslide geometry and kinematics. We find that hydromechanical fatigue is most effective acting in combination with long-term loading and unloading of the slope during glacial cycles. Our results demonstrate that hydromechanical stresses associated with glacial cycles are capable of generating substantial rock slope damage and represent a key preparatory factor for paraglacial slope instabilities.
- Subject:
- Geology
- Creator:
- Moore, Jeffrey, Loew, Simon, Limpach, Philippe, Gischig, Valentin, Grämiger, Lorenz, and Funk, Martin
- Owner:
- Jeff Moore
- Based Near Label Tesim:
- Aletsch Glacier, Valais, Switzerland
- Language:
- English
- Date Uploaded:
- 01/03/2020
- Date Modified:
- 12/09/2021
- Date Created:
- Borehole P1 20130712 09:28:09 to 20140808 09:11:14 and Borehole P2 20130716 05:00:03 to 20140808 22:10:44
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/10.7278/S50D-A50H-3TE4
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- Creator:
- Bromley, Benjamin and Kenyon, Scott
- Owner:
- BENJAMIN BROMLEY
- Language:
- English
- Date Uploaded:
- 07/26/2019
- Date Modified:
- 12/09/2021
- Resource Type:
- Dataset and Software or Program Code
- Identifier:
- https://doi.org/10.7278/S50D-EFCY-ZC00
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- Creator:
- Bromley, Benjamin C. and Kenyon, Scott J.
- Depositor:
- BRIAN MCBRIDE
- Owner:
- BENJAMIN BROMLEY
- Language:
- English
- Date Uploaded:
- 07/11/2019
- Date Modified:
- 12/09/2021
- Date Created:
- 20180515-20181214
- Resource Type:
- Software or Program Code and Dataset
- Identifier:
- https://doi.org/10.7278/s50d-w273-1gg0
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- Description:
- Background: The objective of this study was to evaluate the effect of utilising larger lens cubes on phacoemulsification efficiency and chatter using 3 tips of different sizes and 2 ultrasound (US) approaches. Methods: This was an in vitro laboratory study conducted at the John A. Moran Eye Center Laboratory, University of Utah, Salt Lake City, UT, USA. Porcine lens nuclei were formalin-soaked for 2 hours, then divided into either 2.0 mm or 3.0 mm cubes. 30 degree bent 19 G, 20 G, and 21 G tips were used with a continuous torsional US system; and straight 19 G, 20 G, and 21 G tips were used with a micropulse longitudinal US system. Efficiency and chatter were determined. Results: Mean phacoemulsification removal time was higher with the 3.0 mm lens cube for all US variations and tip sizes. There were statistically significant differences between the 19 G and 21 G tips with micropulse longitudinal US using the 2.0 mm lens cube and the 3.0 mm lens cube, as well as with continuous transversal US using the 2.0 mm lens cube and the 3.0 mm lens cube. There was no significant difference between 19 G and 20 G tips with either lens cube size in either US approach. However, using both US approaches, trends were identical for both lens cube sizes in which the 19 G tips performed better than the 20 G and 21 G tips. Conclusion: Regardless of lens size, the 19 G needle was the most efficient, with the fewest outliers and smallest standard deviations.
- Subject:
- ophthalmology
- Creator:
- Barlow, William R., Bernhisel, Ashlie A., Zaugg, Brian, Olson, Randall J., Ramshekar, Aniket, Heczko, Joshua B., and Pettey, Jeff H.
- Depositor:
- Susan Schulman
- Owner:
- Jeff Pettey
- Based Near Label Tesim:
- Salt Lake City, Utah, United States
- Language:
- English
- Date Uploaded:
- 11/26/2019
- Date Modified:
- 12/09/2021
- Date Created:
- 20180201 - 20180204
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/doi:10.7278/S50D-ZTWP-VF00
-
- Description:
- Localization of the components of the cardiac conduction system (CCS) is essential for many therapeutic procedures in cardiac surgery and interventional cardiology. While histological studies provided fundamental insights into CCS localization, this information is incomplete and difficult to translate to aid in intraprocedural localization. To advance our understanding of CCS localization, we set out to establish a framework for quantifying nodal region morphology. Using this framework, we quantitatively analyzed the sinoatrial node (SAN) and atrioventricular node (AVN) in ovine with menstrual age ranging from 4.4 to 58.3 months. In particular, we studied the SAN and AVN in relation to the epicardial and endocardial surfaces, respectively. Using anatomical landmarks, we excised the nodes and adjacent tissues, sectioned those at a thickness of 4 µm at 100 µm intervals, and applied Masson’s trichrome stain to the sections. These sections were then imaged, segmented to identify nodal tissue, and analyzed to quantify nodal depth and superficial tissue composition. The minimal SAN depth ranged between 20 and 926 µm. AVN minimal depth ranged between 59 and 1192 µm in the AVN extension region, 49 and 980 µm for the compact node, and 148 and 888 µm for the transition to His Bundle region. Using a logarithmic regression model, we found that minimal depth increased logarithmically with age for the AVN (R2=0.818, P=0.002). Also, the myocardial overlay of the AVN was heterogeneous within different regions and decreased with increasing age. Age associated alterations of SAN minimal depth were insignificant. Our study presents examples of characteristic tissue patterns superficial to the AVN and within the SAN. We suggest that the presented framework provides quantitative information for CCS localization. Our studies indicate that procedural methods and localization approaches in regions near the AVN should account for the age of patients in cardiac surgery and interventional cardiology.
- Subject:
- cardiology and physiology
- Creator:
- Frank Sachse, Brian K. Cottle, and Jordan Johnson
- Owner:
- Frank Sachse
- Language:
- English
- Date Uploaded:
- 11/13/2019
- Date Modified:
- 05/17/2023
- Date Created:
- 2018
- Resource Type:
- Dataset
- Identifier:
- https://doi.org/doi:10.7278/S50D-546S-4RVW
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- Description:
- A comprehensive geochemical and stratigraphic study of Cretaceous coal-bearing strata in Utah and western Colorado was performed to evaluate geologic trends in REE-enrichment, as well as elucidate enrichment mechanisms. Preliminary portable X-ray fluorescence (pXRF) analyses (n = 5659) was combined with Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) analyses (n = 135) on particularly REE-enriched samples. Sampling and analyses from active and historic mines as well as nearby cores and outcrops were performed with an emphasis on sedimentary, stratigraphic, geographic, and mining context.
- Keyword:
- critical minerals, Cretaceous period, Mesaverde Group, rare earth elements, energy transition, Ferron Sandstone, Blackhawk Formation, Uinta region, and coal
- Subject:
- economic geology, core analysis, critical minerals , coal resources, nonmetallic mineral resources, sedimentology, and stratigraphy
- Creator:
- Birgenheier, Lauren, Fausett, Peyton, Gall, Ryan, Fernandez, Diego , Giebel, Andrew , Vanden Berg, Michael D., Morris, Emma , Wilcock, Laura , Coe, Haley , and Free, Michael
- Contributor:
- Hoskins, Brittney, Ashurst-McGee, Logan, and Bailey, Nick
- Owner:
- Based Near Label Tesim:
- Salt Lake City, Utah, United States, Uinta Basin, Utah, United States, and Piceance Creek Basin, Colorado, United States
- Language:
- English
- Date Uploaded:
- 12/14/2023
- Date Modified:
- 12/19/2023
- Date Created:
- 2022-05-23 to 2023-08-18
- License:
- Public Domain – This data is free of copyright restrictions (e.g. government sponsored data).
- Resource Type:
- Dataset
- Identifier:
- https://www.doi.org/10.7278/S50d-08s4-3d7j