Search Constraints

Start Over
Filtering by: Creator Brian K. Cottle Remove constraint Creator: Brian K. Cottle Language English Remove constraint Language: English

Search Results

  • Data for: Localization of the Sinoatrial and Atrioventricular Nodal Region in Neonatal and Juvenile Ovine Hearts

    Description:
    Localization of the components of the cardiac conduction system (CCS) is essential for many therapeutic procedures in cardiac surgery and interventional cardiology. While histological studies provided fundamental insights into CCS localization, this information is incomplete and difficult to translate to aid in intraprocedural localization. To advance our understanding of CCS localization, we set out to establish a framework for quantifying nodal region morphology. Using this framework, we quantitatively analyzed the sinoatrial node (SAN) and atrioventricular node (AVN) in ovine with menstrual age ranging from 4.4 to 58.3 months. In particular, we studied the SAN and AVN in relation to the epicardial and endocardial surfaces, respectively. Using anatomical landmarks, we excised the nodes and adjacent tissues, sectioned those at a thickness of 4 µm at 100 µm intervals, and applied Masson’s trichrome stain to the sections. These sections were then imaged, segmented to identify nodal tissue, and analyzed to quantify nodal depth and superficial tissue composition. The minimal SAN depth ranged between 20 and 926 µm. AVN minimal depth ranged between 59 and 1192 µm in the AVN extension region, 49 and 980 µm for the compact node, and 148 and 888 µm for the transition to His Bundle region. Using a logarithmic regression model, we found that minimal depth increased logarithmically with age for the AVN (R2=0.818, P=0.002). Also, the myocardial overlay of the AVN was heterogeneous within different regions and decreased with increasing age. Age associated alterations of SAN minimal depth were insignificant. Our study presents examples of characteristic tissue patterns superficial to the AVN and within the SAN. We suggest that the presented framework provides quantitative information for CCS localization. Our studies indicate that procedural methods and localization approaches in regions near the AVN should account for the age of patients in cardiac surgery and interventional cardiology.
    Subject:
    cardiology and physiology
    Creator:
    Frank Sachse, Brian K. Cottle, and Jordan Johnson
    Owner:
    Frank Sachse
    Language:
    English
    Date Uploaded:
    11/13/2019
    Date Modified:
    05/17/2023
    Date Created:
    2018
    Resource Type:
    Dataset
    Identifier:
    https://doi.org/doi:10.7278/S50D-546S-4RVW