The dataset includes scanning electron micrographs (SEM) and associated instrumentation settings. The data was generated as part of a larger study examining the mouse-strain specific susceptibility to murine cytomegalovirus (mCMV) dependent hearing loss. The included data documents that part of the study that quantified outer hair cell (OHC) loss. The entire study is documented in Almishaal et al. “Natural Killer Cells Attenuate Cytomegalovirus-induced Hearing Loss in Mice” PLoS Pathogens (2017).