This ALLURI_readme20240725.txt file was generated on 20240725 by Rishi Alluri Modified on 20240730 by Kaylee Alexander ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: Whole-cell, In Vivo, Recording Data for Interval Counting Neurons in the Inferior Colliculus of R. pipiens 2. Author Information Principal Investigator Contact Information Name: Rishi K. Alluri Institution: School of Biological Sciences, University of Utah Address: 257 South, 1400 East, Salt Lake City, UT-84112 Email: rishi.alluri@utah.edu ORCID: 0000-0002-4229-927X Associate or Co-investigator Contact Information Name: Gary J. Rose Institution: School of Biological Sciences, University of Utah Address: 257 South, 1400 East, Salt Lake City, UT-84112 Email: rose@bioscience.utah.edu ORCID: 0000-0003-1376-6737 3. Date of data collection (single date, range, approximate date): 20050107 to 20221207 4. Geographic location of data collection (where was data collected?): School of Biological Sciences, University of Utah, Salt Lake City, UT 5. Information about funding sources that supported the collection of the data: National Institute on Deafness and Other Communication Disorders (NIDCD) R01 DC017644 -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY NC - Allows others to use and share your data non-commercially and with attribution. 2. Links to publications that cite or use the data: https://doi.org/10.1073/pnas.2404157121 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for the data: Alluri, Rishi, and Rose, Gary J. 2024. "How Auditory Neurons Count Temporal Intervals and Decode Information." The Hive: University of Utah Research Data Repository. http://doi.org/10.7278/S5d-0ndd-9r1n. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: *.xlsx Short description: Averages of whole-cell membrane potential recordings in vivo from the anuran inferior colliculus and approximated parameters for estimating excitatory and inhibitory conductances. B. Filename: *.m Short description: MATLAB files for analysis of data in *.xlsx files. C. Filename: *.s2s Short description: CED Spike2 scripts for processing acquired .smr and .smrx files using CED Power 1401. 2. Relationship between files: .xlsx: These files contain averages of whole-cell recording data; the values of the average at each time point are along the columns along with the current clamp at which they were recorded (1st row). Additionally, the approximate parameters required for the analysis of conductance. .m: Matlab files contain code to estimate the excitatory and inhibitory conductances at each point in time, as provided in the .xlsx files. Additionally, the mean and net conductances are computed. .s2s: CED Spike2 scripts were used to generate the averages of whole-cell recordings (membrane potential traces). 3. Additional related data collected that was not included in the current data package: Some of the data was with-held for use in a manuscript we are preparing. That data will be released along with the manuscript when it is ready for publication. 4. Are there multiple versions of the dataset? No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Data collection methods are described in the following papers: G. J. Rose, R. K. Alluri, G. A. Vasquez-Opazo, S. E. Odom, J. A. Graham, and C. J. Leary. 2013. "Combining pharmacology and whole-cell patch recording from CNS neurons, in vivo." Journal of Neuroscience Methods 213(1): 99–104. https://doi.org/10.1016/j.jneumeth.2012.12.003. R. K. Alluri, G. J. Rose, J. L. Hanson, C. J. Leary, G. A. Vasquez-Opazo, J. A. Graham, and J. Wilkerson. 2016. "Phasic, suprathreshold excitation and sustained inhibition underlie neuronal selectivity for short-duration sounds." PNAS 113(13): E1927-E1935. https://doi.org/10.1073/pnas.1520971113. R. K. Alluri, G. J. Rose, C. J. Leary, A. Palaparthi, J. L. Hanson, G. A. Vasquez-Opazo, J. A. Graham, and K. Long. 2021. "How auditory selectivity for sound timing arises: The diverse roles of GABAergic inhibition in shaping the excitation to interval-selective midbrain neurons." Progress in Neurobiology 199: 101962. https://doi.org/10.1016/j.pneurobio.2020.101962 2. Methods for processing the data: The *.xlsx files consist of averaged (per stimulus) whole-cell, in vivo, recordings. These are made from raw CED Spike2 smr & smrx files using spike2 scripting language. 3. Instrument- or software-specific information needed to interpret the data: MATLAB, Excel 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: NA 6. Describe any quality-assurance procedures performed on the data: NA 7. People involved with sample collection, processing, analysis and/or submission: Gustavo A. Vasquez-Opazo, Stephen E. Odom, Jalina A. Graham, Christopher J. Leary, Jessica L. Hanson, Jeremy Wilkerson, Anil Palaparthi, Kyphuong Luong, Jamie McDowell, Anwesha Mukhopadhyay ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [FILENAME] ----------------------------------------- 1. Number of variables: 2. Number of cases/rows: Each column is an averaged current clamp recording indicated by the amount of current injected in the first row. Additionally, simulus and representative trace of the average are presented in some files. 3. Variable List A. Name: Iinj Description: Injected current for current clamp recording. B. Name: Cm Description: Membrane Conductance, measured using double exponential fit. See citation methods. C. Name: Rin Description: Input Resistance, measured using double exponential fit. See citation methods. D. Name: Er Description: The resting potential of the neuron, usually the steady state potential when no current is injected. E. Name: Ee Description: Excitatory reversal potential, the potential at which depolarizations go to zero. F. Name: Ei Description: Inhibitory reversal potential, the potential at which depolarizations go to zero. G. Name: Et Description: Threshold potential of the neuron. H. Name: Eact Description: Estimated activation potential of the neuron to tune-out/filter active currents. I. Name: xalpha Description: Tuning multiplier on the active conductance parameter, alpha. J. Name: xbeta Description: Tuning multiplier on the active conductance parameter, alpha. K. Name: sps Description: Spikes per stimulus presentation. L. Name: Eref Description: Reference potential to measure the amount of depolarization and hyper-polarization. M. Name: rate Description: The rate at which sound pulses are presented acoustically (Available upon request from the authors if not present in the xlsx file). N. Name: npulses Description: Number of sound pulses presented in the acoustic stimulus (Available upon request from the authors if not present in the xlsx file). O. Name: pd Description: Pulse duration (sec) of each presented acoustic pulse (Available upon request from the authors if not present in the xlsx file). P. Name: fc Description: Carrier frequency in Hz used to generate each sound pulse (Available upon request from the authors if not present in the xlsx file). Q. Name: spl Description: Sound pressure level of the acoustic stimulus (Available upon request from the authors if not present in the xlsx file). R. Name: speaker Description: The speaker used to deliver the sound pulses (Available upon request from the authors if not present in the xlsx file). 4. Missing data codes: NA 5. Specialized formats of other abbreviations used: NA