This MUKHOPADHYAY_readme20250827.txt file was generated on 20250827 by Kaylee Alexander ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Chorus Frog Population LIN Spikes per Stimulus Presentation Data 2. Author Information Principal Investigator Contact Information Name: Anwesha Mukhopadhyay Institution: School of Biological Sciences, University of Utah Address: 257 South 1400 East, 201 Salt Lake City, UT 84112 Email: anwesha.mukhopadhyay@utah.edu ORCID: 0009-0004-0704-7001 Associate or Co-investigator Contact Information Name: Vinayak G. Kamath Institution: School of Biological Sciences, University of Utah Address: 257 South 1400 East, 201 Salt Lake City, UT 84112 Email: vinayak.gopalakrishnakamath@utah.edu ORCID: 0000-0001-7645-0831 Associate or Co-investigator Contact Information Name: Jamie M. McDowell Institution: Department of Psychology, University of California, Los Angeles Email: jamiemcd@ucla.edu ORCID: 0009-0005-2720-6978 Associate or Co-investigator Contact Information Name: Auburn S. Acord Institution: Neuroscience Program, University of Utah ORCID: 0009-0005-1786-8353 Associate or Co-investigator Contact Information Name: Rishi K. Alluri Institution: School of Biological Sciences, University of Utah Address: 257 South 1400 East, 201 Salt Lake City, UT 84112 ORCID: 0000-0002-4229-927X Associate or Co-investigator Contact Information Name: Gary J. Rose Institution: School of Biological Sciences, University of Utah Address: 257 South 1400 East, 201 Salt Lake City, UT 84112 ORCID: 0000-0003-1376-6737 Associate or Co-investigator Contact Information Name: Emily M. Lemmon Institution: Department of Biological Sciences, Florida State University ORCID: 0000-0001-5911-6102 3. Date of data collection (single date, range, approximate date): 20220211 to 20250611 4. Geographic location of data collection (where was data collected?): School of Biological Sciences, University of Utah 5. Information about funding sources that supported the collection of the data: NSF DEB-2121058; NIH R01 DC017466-01; NSF DEB-2513022 -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY NC - Allows others to use and share your data non-commercially and with attribution. 2. Links to publications that cite or use the data: NA 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for the data: Mukhopadhyay, Anwesha, Vinayak G. Kamath, Jamie M. McDowell, Auburn S. Acord, Rishi K. Alluri, and Gary J. Rose. 2025. "Chorus Frog Population LIN Spikes per Stimulus Presentation Data." The Hive: University of Utah Research Data Repository. [DOI]. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: 20250821_LIN-Allopatric-AL_Mukhopadhyay.csv Short description: This dataset contains extracellular neurophysiological recordings from long interval neurons in the auditory midbrain of Pseudacris feriarum frogs collected from an allopatric population in Alabama. Frogs were presented with synthetic acoustic stimuli varying in pulse rate (5–70 pulses/s) to assess pulse rate selectivity. Data include neuronal responses to acoustic stimuli. The purpose of the recordings was to compare neural response to stimuli between allopatric and sympatric populations. B. Filename: 20250821_LIN-Allopatric-GA_Mukhopadhyay.csv Short description: This dataset contains extracellular neurophysiological recordings from long interval neurons in the auditory midbrain of Pseudacris feriarum frogs collected from an allopatric population in Georgia. Frogs were presented with synthetic acoustic stimuli varying in pulse rate (5–70 pulses/s) to assess pulse rate selectivity. Data include neuronal responses to acoustic stimuli. The purpose of the recordings was to compare neural response to stimuli between allopatric and sympatric populations. C. Filename: 20250821_LIN-Sympatric-GA_Mukhopadhyay.csv Short description: This dataset contains extracellular neurophysiological recordings from long interval neurons in the auditory midbrain of Pseudacris feriarum frogs collected from a sympatric population in Georgia. Frogs were presented with synthetic acoustic stimuli varying in pulse rate (5–70 pulses/s) to assess pulse rate selectivity. Data include neuronal responses to acoustic stimuli. The purpose of the recordings was to compare neural response to stimuli between allopatric and sympatric populations. D. Filename: 20250821_LIN-Sympatric-SC_Mukhopadhyay.csv Short description: This dataset contains extracellular neurophysiological recordings from long interval neurons in the auditory midbrain of Pseudacris feriarum frogs collected from a sympatric population in South Carolina. Frogs were presented with synthetic acoustic stimuli varying in pulse rate (5–70 pulses/s) to assess pulse rate selectivity. Data include neuronal responses to acoustic stimuli. The purpose of the recordings was to compare neural response to stimuli between allopatric and sympatric populations. 2. Relationship between files: NA 3. Additional related data collected that was not included in the current data package: NA 4. Are there multiple versions of the dataset? No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Stimulus Generation and Presentation: To test pulse rate selectivity of ICan neurons across populations, stimuli were generated with pulses that represented natural pulse shape and duration. The frogs across populations were presented with synthetic stimuli of 10 pulses representative of natural call pulse shapes containing PRs varying from 5 to 70 pps. Acoustic stimuli were generated using MATLAB and Tucker Davis Technologies (TDT) System III and presented free field in an Industrial Acoustics audiometric room (87" x 87" x 78" high). The loudspeakers (Bose 101) are situated 0.5 meters from the frog, contralateral and ipsilateral to the recording site and orthogonal to each other. Reflections in the booth are minimized by acoustic foam on the walls and ceiling (absorption coefficients from 250-4000 Hz: 0.60-0.97). Stimuli, therefore, are presented at levels not exceeding 30 dB SPL above each unit's threshold. A microphone (ACO Pacific, with Cetec Ivie IE-2P preamp) is used to measure stimulus levels (re. 20 µPa) via a sound level meter (Cetec Ivie IE-30A). Neurophysiology: Microelectrodes for extracellular recording are constructed from borosilicate glass (1.0 mm OD, 0.58 mm ID) on a Brown-Flaming (model P-97) puller and have tip diameters of approximately 1 μm. These micropipettes are filled with 2M NaCl and have resistances of ~1-1.5MΩ. The recording pipettes will be advanced into the brain using a 3-axis microdrive (Scientifica PLC, model IVM-3000). All procedures were conducted in compliance with the Society of Neuroscience’s Policy on the Use of Animals in Neuroscience Research and the National Institutes of Health guidelines and are approved by the University of Utah’s Institutional Animal Care and Use Committee. 2. Methods for processing the data: Spike responses were averaged across trials. All spike analyses were performed in Spike2 v10. 3. Instrument- or software-specific information needed to interpret the data: Data are provided as Excel (.xlsx) files, which include neuronal responses to synthetic pulse rate stimuli. Microsoft Excel (or any compatible spreadsheet software) can be used to open and analyze these files. 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: Acoustic stimuli presented free field in an Industrial Acoustics audiometric room (87" x 87" x 78" high). The loudspeakers (Bose 101) are situated 0.5 meters from the frog, contralateral and ipsilateral to the recording site and orthogonal to each other. Reflections in the booth are minimized by acoustic foam on the walls and ceiling (absorption coefficients from 250-4000 Hz: 0.60-0.97). The temperature of the frog and booth is maintained at 18 and 20 degrees centigrade. 6. Describe any quality-assurance procedures performed on the data: Only recordings with clear, well isolated spikes were included in the dataset. 7. People involved with sample collection, processing, analysis and/or submission: NA ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20250821_LIN-Allopatric-AL_Mukhopadhyay.csv ----------------------------------------- 1. Number of variables: 17 2. Number of cases/rows: 106 3. Variable List A. Name: Frog ID Description: The ID of each specimen used B. Name: Recording Date Description: The date of recording C. Name: Neuron # Description: nth neuron recorded that date D. Name: 5pps Description: spikes per stimulus presentation at 5 pulses per second. E. Name: 10pps Description: spikes per stimulus presentation at 10 pulses per second. F. Name: 15pps Description: spikes per stimulus presentation at 15 pulses per second. G. Name: 20pps Description: spikes per stimulus presentation at 20 pulses per second. H. Name: 25pps Description: spikes per stimulus presentation at 25 pulses per second. I. Name: 30pps Description: spikes per stimulus presentation at 30 pulses per second. J. Name: 35pps Description: spikes per stimulus presentation at 35 pulses per second. K. Name: 40pps Description: spikes per stimulus presentation at 40 pulses per second. L. Name: 45pps Description: spikes per stimulus presentation at 45 pulses per second. M. Name: 50pps Description: spikes per stimulus presentation at 50 pulses per second. N. Name: 55pps Description: spikes per stimulus presentation at 55 pulses per second. O. Name: 60pps Description: spikes per stimulus presentation at 60 pulses per second. P. Name: 65pps Description: spikes per stimulus presentation at 65 pulses per second. Q. Name: 70pps Description: spikes per stimulus presentation at 70 pulses per second. 4. Missing data codes: NA 5. Specialized formats of other abbreviations used: NA ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20250821_LIN-Allopatric-GA_Mukhopadhyay ----------------------------------------- 1. Number of variables: 17 2. Number of cases/rows: 47 3. Variable List A. Name: Frog ID Description: The ID of each specimen used B. Name: Recording Date Description: The date of recording C. Name: Neuron # Description: nth neuron recorded that date D. Name: 5pps Description: spikes per stimulus presentation at 5 pulses per second. E. Name: 10pps Description: spikes per stimulus presentation at 10 pulses per second. F. Name: 15pps Description: spikes per stimulus presentation at 15 pulses per second. G. Name: 20pps Description: spikes per stimulus presentation at 20 pulses per second. H. Name: 25pps Description: spikes per stimulus presentation at 25 pulses per second. I. Name: 30pps Description: spikes per stimulus presentation at 30 pulses per second. J. Name: 35pps Description: spikes per stimulus presentation at 35 pulses per second. K. Name: 40pps Description: spikes per stimulus presentation at 40 pulses per second. L. Name: 45pps Description: spikes per stimulus presentation at 45 pulses per second. M. Name: 50pps Description: spikes per stimulus presentation at 50 pulses per second. N. Name: 55pps Description: spikes per stimulus presentation at 55 pulses per second. O. Name: 60pps Description: spikes per stimulus presentation at 60 pulses per second. P. Name: 65pps Description: spikes per stimulus presentation at 65 pulses per second. Q. Name: 70pps Description: spikes per stimulus presentation at 70 pulses per second. 4. Missing data codes: NA 5. Specialized formats of other abbreviations used: NA ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20250821_LIN-Sympatric-GA_Mukhopadhyay ----------------------------------------- 1. Number of variables: 17 2. Number of cases/rows: 67 3. Variable List A. Name: Frog ID Description: The ID of each specimen used B. Name: Recording Date Description: The date of recording C. Name: Neuron # Description:nth neuron recorded that date D. Name: 5pps Description: spikes per stimulus presentation at 5 pulses per second. E. Name: 10pps Description: spikes per stimulus presentation at 10 pulses per second. F. Name: 15pps Description: spikes per stimulus presentation at 15 pulses per second. G. Name: 20pps Description: spikes per stimulus presentation at 20 pulses per second. H. Name: 25pps Description: spikes per stimulus presentation at 25 pulses per second. I. Name: 30pps Description: spikes per stimulus presentation at 30 pulses per second. J. Name: 35pps Description: spikes per stimulus presentation at 35 pulses per second. K. Name: 40pps Description: spikes per stimulus presentation at 40 pulses per second. L. Name: 45pps Description: spikes per stimulus presentation at 45 pulses per second. M. Name: 50pps Description: spikes per stimulus presentation at 50 pulses per second. N. Name: 55pps Description: spikes per stimulus presentation at 55 pulses per second. O. Name: 60pps Description: spikes per stimulus presentation at 60 pulses per second. P. Name: 65pps Description: spikes per stimulus presentation at 65 pulses per second. Q. Name: 70pps Description: spikes per stimulus presentation at 70 pulses per second. 4. Missing data codes: NA 5. Specialized formats of other abbreviations used: NA ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20250821_LIN-Sympatric-SC_Mukhopadhyay ----------------------------------------- 1. Number of variables: 17 2. Number of cases/rows: 89 3. Variable List A. Name: Frog ID Description: The ID of each specimen used B. Name: Recording Date Description: The date of recording C. Name: Neuron # Description: nth neuron recorded that date D. Name: 5pps Description: spikes per stimulus presentation at 5 pulses per second. E. Name: 10pps Description: spikes per stimulus presentation at 10 pulses per second. F. Name: 15pps Description: spikes per stimulus presentation at 15 pulses per second. G. Name: 20pps Description: spikes per stimulus presentation at 20 pulses per second. H. Name: 25pps Description: spikes per stimulus presentation at 25 pulses per second. I. Name: 30pps Description: spikes per stimulus presentation at 30 pulses per second. J. Name: 35pps Description: spikes per stimulus presentation at 35 pulses per second. K. Name: 40pps Description: spikes per stimulus presentation at 40 pulses per second. L. Name: 45pps Description: spikes per stimulus presentation at 45 pulses per second. M. Name: 50pps Description: spikes per stimulus presentation at 50 pulses per second. N. Name: 55pps Description: spikes per stimulus presentation at 55 pulses per second. O. Name: 60pps Description: spikes per stimulus presentation at 60 pulses per second. P. Name: 65pps Description: spikes per stimulus presentation at 65 pulses per second. Q. Name: 70pps Description: spikes per stimulus presentation at 70 pulses per second. 4. Missing data codes: NA 5. Specialized formats of other abbreviations used: NA