This KAMATH_readme20250820.txt file was generated on 20250820 by Kaylee Alexander ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Data Supporting 'Evidence That Interval-Counting Neurons Play a Critical Role in Call Recognition by Cope’s Gray Treefrogs' 2. Author Information Principal Investigator Contact Information Name: Vinayak G. Kamath Institution: University of Utah, School of Biological Sciences Address: 257 South 1400 East, Rm 201, Salt Lake City, Utah, 84112 Email: vinayak.gopalakrishnakamath@utah.edu ORCID: 0000-0001-7645-0831 3. Date of data collection (single date, range, approximate date)  20240501 to 20250815 4. Geographic location of data collection (where was data collected?): Tamarack Nature Center, Ramsey County, Minnesota (behavioral phonotaxis collection); University of Minnesota – Twin Cities, St. Paul, MN (behavioral experiments); University of Utah, Salt Lake City, UT (neurophysiological recordings) 5. Information about funding sources that supported the collection of the data: National Institute on Deafness and Other Communication Disorders (NIDCD) R01 DC017466; NIDCD R21 DC022648; National Science Foundation (NSF) 2022253 -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY NC – Allows others to use and share your data non-commercially and with attribution. 2. Links to publications that cite or use the data: NA 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for the data: Kamath, Vinayak G. 2025. "Data Supporting 'Evidence That Interval-Counting Neurons Play a Critical Role in Call Recognition by Cope’s Gray Treefrogs.'" The Hive: University of Utah Research Data Repository. https://doi.org/10.7278/S5d-7mt5-z18h. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: 20250815_ExtracellularICN_LIN_Kamath.xlsx Short description: Contains behavioral phonotaxis outcomes for each individual Hyla chrysoscelis female tested in 2024. The Score sheet includes binary response data (1 = positive phonotaxis; 0 = no response) across five stimulus conditions (1X, MI=2X, MI=3X, AI=2X, AI=3X B. Filename: 20240815_MixedIntervalPhonotaxis_Kamath.xlsx Short description: Contains extracellular neurophysiological recordings from three neuron classes in the midbrain auditory nucleus (ICN, LIN, Nonsel). Each sheet corresponds to one neuron type and lists individual neurons (with date, neuron ID, Best excitatory frequency (BEF), pulse rate, pulse number threshold for ICNs, and gap pulse number), along with spike responses per stimulus presentation across varying temporal paradigms (1X, MI=2X–7X, AI=2X–7X). 2. Relationship between files: NA 3. Additional related data collected that was not included in the current data package: NA 4. Are there multiple versions of the dataset? No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Data in 20240815_MixedIntervalPhonotaxis_Kamath.xlsx were collected using standard single-stimulus behavioral phonotaxis assays in a hemi-anechoic chamber. Gravid females of Hyla chrysoscelis were tested for stimulus attractiveness to synthetic call stimuli with systematically altered interpulse interval (IPI) structure. Methods follow established procedures (Gerhardt 1995; Bee & Schwartz 2009; LaBarbera et al. 2020). The data in 20250815_ExtracellularICN_LIN_Kamath.xlsx were collected using in vivo single-cell extracellular recordings from midbrain auditory neurons in Hyla chrysoscelis. Stimuli were synthetic call-like trains with controlled IPI structure similar to the behavioral experiment. Methods follow Rose et al. (2015), Hanson et al. (2016). 2. Methods for processing the data: Behavioral data: Raw trial outcomes (approach/no approach) were directly coded as binary values (1 = positive phonotaxis; 0 = no response). No further transformations were applied; Neurophysiology data: Spike counts per stimulus presentation were recorded in Spike2 software, averaged across trials, and normalized to each neuron’s maximum response across conditions. ICNs were classified by pulse-number threshold (≥ 4 pulses for inclusion) and their best rate. LINs and nonselective neurons were classified based on response properties. 3. Instrument- or software-specific information needed to interpret the data: Behavioral stimuli were generated in MATLAB (R2024b) and played back via MOTU 16A soundcard and Crown XLS 1000 amplifiers through Mod1 Orb speakers. Neurophysiology recordings used Tucker-Davis Technologies (TDT) Systems II & III hardware, digitized via Cambridge Electronic Design (CED) Power 1401 interface, and analyzed in Spike2 (v10). Data sheets are standard .xlsx files and can be opened in Microsoft Excel, R, Python (pandas), or other spreadsheet-compatible programs. 4. Standards and calibration information, if appropriate: Acoustic playback levels were calibrated to 85 dB SPL (re: 20 µPa) at 1 m using a Brüel & Kjær sound level meter. No other external standards or calibration were required for spike normalization procedures. 5. Environmental/experimental conditions: Behavioral tests: conducted at 20 ± 1°C in a hemi-anechoic chamber under infrared illumination; Neurophysiology: recordings conducted in an audiometric chamber maintained at 20 ± 2°C. 6. Describe any quality-assurance procedures performed on the data: Only females verified as H. chrysoscelis by two-choice species discrimination tests were included. Unresponsive individuals were retested with conspecific calls to confirm motivation. For neurophysiology, only stable single-unit recordings with clear spike isolation were included. Responses were normalized within neuron to account for firing rate variability. All statistical analyses were conducted using R (v4.3), with appropriate corrections for multiple comparisons. 7. People involved with sample collection, processing, analysis and/or submission: Anwesha Mukhopadhyay, Auburn Sage Acord, Gary J. Rose, Mark A. Bee, and Rishi K. Alluri. Members of the Bee Lab (University of Minnesota) assisted with animal collection during the 2024 breeding season, including undergraduate researchers. Collection of gray treefrogs was conducted under permits issued by the Minnesota Department of Natural Resources, with after-hours field access provided by the Ramsey County Department of Parks and Recreation. Coding of mixed-interval acoustic stimuli was supported by James Steele (Rose Lab, University of Utah). ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20240815_MixedIntervalPhonotaxis_Kamath.xlsx ----------------------------------------- 1. Number of variables: 6 2. Number of cases/rows: 20 3. Variable List A. Name: FROG_ID Description: Unique identifier for each female Hyla chrysoscelis tested in the behavioral phonotaxis assay. B. Name: 1X Description: Binary response (1 = positive phonotaxis, 0 = no response) to the standard conspecific advertisement call (all interpulse intervals = 20 ms). C. Name: MI=2X Description: Binary response (1 = positive phonotaxis, 0 = no response) to stimulus in which the 5th interpulse interval was doubled (40 ms). D. Name: MI=3X Description: Binary response (1 = positive phonotaxis, 0 = no response) to stimulus in which the 5th interpulse interval was tripled (60 ms). E. Name: AI=2X Description: Binary response (1 = positive phonotaxis, 0 = no response) to stimulus in which interpulse intervals alternated between the standard (20 ms) and doubled (40 ms) values. F. Name: AI=3X Description: Binary response (1 = positive phonotaxis, 0 = no response) to stimulus in which interpulse intervals alternated between the standard (20 ms) and tripled (60 ms) values. 4. Missing data codes: NA MI = Middle Interval AI = Alternating Interval ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: 20250815_ExtracellularICN_LIN_Kamath.xlsx ----------------------------------------- 1. Number of variables: ICN: 15 LIN: 19 Nonsel: 14 2. Number of cases/rows: ICN: 29 LIN: 32 Nonsel: 8 3. Variable List A. Name: Date Description: Date of recording session (YYYY-MM-DD). B. Name: Neuron_number Description: Identifier for the neuron within that recording session. C. Name: Frequency Description: Best excitatory frequency of the neuron (Hz). D. Name: Neuron_type Description: Neuron classification: ICN = Interval-Counting Neuron; LIN = Long-Interval Neuron; Nonsel = Non-selective neuron. E. Name: Pulse_rate Description: Pulse rate of the stimulus (pulses/second, pps). F. Name: Pulse_number_threshold Description: ICN sheet only. Minimum number of pulses at optimal IPI required to elicit spiking (PNT). G. Name: Type Description: ICN sheet only. ICN subclassification (e.g., BP = band-pass). H. Name: Gap_pulse_number Description: Total number of pulses in the tested stimulus (typically 8–10). I. Name: 1X Description: Average normalized spike response to the standard stimulus. I. Name: MI=2X […] MI=7X Description: Average normalized spike response when the middle IPI was lengthened to 2×, 3× […] 7× the standard. K. Name: AI=2X […] AI=7X Description: Average normalized spike response when IPIs alternated between the standard and a longer value (2×, 3× […] 7×). 4. Missing data codes: [blank] Missing data 5. Specialized formats of other abbreviations used: ICN = Interval-Counting Neuron LIN = Long-Interval Neuron Nonsel = Non-selective neuron IPI = Interpulse Interval MI = Middle Interval AI = Alternating Interval PNT = Pulse Number Threshold BP = Band-pass