This SZULIK_readme20250528.txt file was generated on 20250528 by Kaylee Alexander and updated on 20251030 by Madison Golden ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Histone H4K20 Methylation and its Regulating Enzymes in the Heart 2. Author Information Principal Investigator Contact Information Name: Sarah Franklin Institution: CVRTI, University of Utah Address: 95 S 2000 E Rm 103, Salt Lake City, UT 84112 Email: sarah.franklin@utah.edu ORCID: 0000-0002-2958-1879 Associate or Co-investigator Contact Information Name: Marta Szulik Institution: CVRTI, University of Utah Address: 95 S 2000 E Rm 103, Salt Lake City, UT 84112 Email: m.szulik@utah.edu ORCID: 0000-0001-5412-8219 Associate or Co-investigator Contact Information Name: Samuel Hickenlooper Institution: CVRTI, University of Utah Address: 95 S 2000 E Rm 103, Salt Lake City, UT 84112 ORCID: 0000-0003-1535-7179 3. Date of data collection (single date, range, approximate date): 2015 to 2023 4. Geographic location of data collection (where was data collected?): Salt Lake City, UT 5. Information about funding sources that supported the collection of the data: This work was supported by funding from the following sources: the National Institutes of Health (NIH) grants from the National Heart, Lung and Blood Institute: R01-HL-130424 (S.F.), R01-HL-161045 (S.F.), and the Ruth L. Kirschstein National Research Service Award T32HL007576 (J.R.V.). Next, we thank the Nora Eccles Harrison Treadwell Foundation Grants (S.F.) and the H.A. and Edna Benning Society at the University of Utah for funding contributed to S.G.D. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY NC - Allows others to use and share your data non-commercially and with attribution. 2. Links to publications that cite or use the data: "Expression profiles of histone H4K20 methylation and its associated enzymes in mouse cardiac disease and human heart failure" DOI: Forthcoming 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for the data: Franklin, Sarah, Marta Szulik, and Samuel Hickenlooper. 2025. "Histone H4K20 Methylation and its Regulating Enzymes in the Heart." The Hive: University of Utah Research Data Repository. https://doi.org/10.7278/S5d-h2nc-qa57. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Szulik_H4K20me_05032025.xlsx Short description: Excel file containing 1 sheet per graph in the related manuscript, "Differential Regulation of Histone H4K20 Methylation and Its Associated Enzymes in Mouse Cardiac Disease and Human Heart Failure." Each sheet is labeled according to the figure numbers and contains the raw data used to generate the figure. B. Filename: Szulik_SupplementalData_05032025.pdf Short description: PDF file containing supplementary tables that are included as supplementary material to the manuscript. It includes primer sequences as well as tables with clinical characterization of human subjects who provided samples to generate data presented in this manuscript. 2. Relationship between files: NA 3. Additional related data collected that was not included in the current data package: NA 4. Are there multiple versions of the dataset? No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Histone H4K20 di and tri-methylation abundance as well as expression of 12 methyltransferases and demethylases was examined across various models: one cell model, two mouse models of cardiac dysfunction, and cardiac tissue from heart failure patients. Specifically, we used H9c2 cells treated with phenylephrine (PE) to induce acute hypertrophy in isolated cardiomyocytes. In mouse models, we analyzed cardiac tissue from mice treated with isoproterenol (ISO), a β-adrenergic receptor agonist known to induce cardiac hypertrophy, as well as from mice subjected to ischemic injury via ligation of the left anterior descending (LAD) artery to model heart failure. To assess relevance to human health, we analyzed heart tissue samples from patients with ischemic cardiomyopathy (ICM) and compared them to donor controls. In addition, we also analyzed samples from patients undergoing LVAD implantation as a bridge to heart transplant. Clinical characteristics of the study population are included in supplementary tables S2-S4. 2. Methods for processing the data: Gene expression was measured using quantitative real-time PCR, while protein levels were assessed by electrophoresis followed by western blotting. Cardiac function was evaluated by echocardiography-measuring left ventricular ejection fraction (LVEF) in mice and both LVEF and left ventricular end-diastolic diameter (LVEDD) in humans-and by heart weight to body weight (HW/BW) ratios in mouse models. For samples from LVAD patients and healthy controls, we utilized RNA-Seq data previously reported by Drakos et al (Circulation, 2023, DOI: 10.1161/CIRCULATIONAHA.121.056600). That study compared transcripts with fold change greater than or equal to 1.5 and a p-value of 0.05. From the unpublished portion of this data set, we extracted log2 values of six methyltransferases (KMT5A, MMSET, KMT7B, KMT5C, SMYD3, and SMYD5), and six demethylases (KDM7B, KDM9, KDM1A, RAD23A, RAD23B, and KDM7C) to analyse their expression in failing hearts (both responders and non-responders) versus healthy controls. 3. Instrument- or software-specific information needed to interpret the data: Microsoft Excel or other software that reads .xlsx file. 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: Cameron Brady, Ryan Bia, Joseph R. Visker, Li Wang, Steven Valdez, Clint Gwynn, Maya N. Roland, Christos P. Kyriakopoulos, Konstantinos Sideris, Stavros G. Drakos ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Szulik_H4K20me_05032025.xlsx ----------------------------------------- 1. Number of cases: 30, each represents the data informing the figures in the related manuscript. 2. Missing data codes: NA 3. Specialized formats of other abbreviations used: NA